Label each TLC plate with standard vs. experim of the protocol (be specific) the TLC was recorded. a. ental spots, what compound corresponds to each spot, and during which step b. In the space below, calculate the R values for each of the three compounds of the standard mixture provided by the teaching assistant as well s any spots) seen for ...
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Most standard TLC plates have ~250 micron stationary phase, these plates have a thicker stationary phase, for prep use. So whatever spots are missing or have become very faint (from incomplete neutralization of the amine) when comparing the two samples are very likely amines (or compounds...
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A sample was spotted on two different TLC plates:a) One plate was placed in a chamber containing methanol, and after developed it showed one spot with an Rf value of 0.97. The other plate was placed in a chambercontaining hexane, and after developed it showed two spots, one with an Rf value of 0.54 and one with an Rf value of 0.37.
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You can, though, work out the R f values for each of the spots in both solvents, and then compare these with values that you have measured for known compounds under exactly the same conditions. How does paper chromatography work? Although paper chromatography is simple to do, it is quite difficult to explain compared with thin layer chromatography.
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